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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in lots of tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two added splice variants were being documented but thorough nucleotide sequence information and facts is not really nonetheless offered.
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Blocking peptides are peptides that bind precisely towards the concentrate on antibody and block antibody binding. These peptide commonly consists of the epitope identified with the antibody.
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to make sure the absolute best success are...
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This tutorial outlines further more achievable optimization of mobile and immunoassay parameters to ensure the absolute best benefits are...
Antibodies bound to the blocking peptide no longer bind towards the epitope about the focus on protein. By comparing the staining in the blocked antibody compared to the antibody by yourself, one can see which staining is particular.
Concentrate on Facts Cyclin E belongs on the remarkably conserved cyclin family members, whose associates are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation styles which lead to your temporal coordination of each and every mitotic function. Cyclin E sorts a posh with and functions like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is also degraded as cells development by S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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